Multifunctional CARS (Coherent Anti-Stokes Raman Spectroscopy) Imaging

CARS microscopy has been used to visualize living cells with contrast of different vibrational modes, including the phosphate stretch vibration (DNA), amide I vibration (protein), OH stretching vibration (water), and the CH group of stretching vibrations (lipids). Due to its nonlinear optical effect, CARS is much more efficient than spontaneous Raman spectroscopy, enabling faster, more sensitive analyses with less photo exposure. Moreover, CARS circumvents the need for extrinsic labels, allowing observation of dynamic, phenomena for which tags are not available. Further work will be focused on measurement of different samples, imaging multimodality and CARS spectroscopy development, spatial resolution improvement for measurement of nanomaterial.



Department of Electrical and Computer Engineering
University of Nebraska-Lincoln
209N Scott Engineering Center
P.O. Box 880511
Lincoln, NE 68588-0511, USA


Phone: (402) 472-3771
Fax:     (402) 472-4732



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